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Monitoring Thiopurine Metabolites in Korean Pediatric Patients with Inflammatory Bowel Disease

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All nine exons of the TPMT gene were amplified on a model 9700 thermal cycler (Applied Biosystems, Foster City, CA, USA) using oligonucleotide primers and conditions as previously described with some modifications.²⁰^,²¹Direct sequencing was performed using ABI Big Dye Terminator kit v1.1 (Applied Biosystems) according to the manufacturer's instructions. To identify sequence variations, patient sequences were compared with respective reference sequences using the Sequenchersoftware (Gene Codes Corporation, Ann Arbor, MI, USA).

Monthly Publications Archive

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Monitoring Thiopurine Metabolites in Korean Pediatric Patients with Inflammatory Bowel Disease

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Monthly Publications Archive

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Monitoring Thiopurine Metabolites in Korean Pediatric Patients with Inflammatory Bowel Disease

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